Mitragynine inhibits the COX-2 mRNA expression and prostaglandin E2 production induced by lipopolysaccharide in RAW264.7 macrophage cells.
J Ethnopharmacol
; 136(1): 75-82, 2011 Jun 14.
Article
em En
| MEDLINE
| ID: mdl-21513785
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE [corrected] Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties in its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. MATERIALS AND METHODS:
The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E(2) (PGE(2)) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE(2) production was quantified using Parameter™ PGE(2) Assay (R&D Systems).RESULTS:
Mitragynine produced a significant inhibition on the mRNA expression of COX-2 induced by LPS, in a dose dependent manner and this was followed by the reduction of PGE(2) production. On the other hand, the effects of mitragynine on COX-1 mRNA expression were found to be insignificant as compared to the control cells. However, the effect of mitragynine on COX-1 protein expression is dependent on concentration, with higher concentration of mitragynine producing a further reduction of COX-1 expression in LPS-treated cells.CONCLUSIONS:
These findings suggest that mitragynine suppressed PGE(2) production by inhibiting COX-2 expression in LPS-stimulated RAW264.7 macrophage cells. Mitragynine may be useful for the treatment of inflammatory conditions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Dinoprostona
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Mitragyna
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Alcaloides de Triptamina e Secologanina
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Ciclo-Oxigenase 2
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Inibidores de Ciclo-Oxigenase 2
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Anti-Inflamatórios
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article