A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite.

In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (k(s)) was calculated to be 270.6s⁻¹. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5 × 10⁻6molL⁻¹ to 30×10⁻6molL⁻¹, with a detection limit of 0.37 × 10⁻6molL⁻¹ (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (K(m)(app)) was 77.7 µmolL⁻¹. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.