Multivalent display of proteins on viral nanoparticles using molecular recognition and chemical ligation strategies.
Biomacromolecules
; 12(6): 2293-301, 2011 Jun 13.
Article
em En
| MEDLINE
| ID: mdl-21545187
ABSTRACT
Multivalent display of heterologous proteins on viral nanoparticles forms a basis for numerous applications in nanotechnology, including vaccine development, targeted therapeutic delivery, and tissue-specific bioimaging. In many instances, precise placement of proteins is required for optimal functioning of the supramolecular assemblies, but orientation- and site-specific coupling of proteins to viral scaffolds remains a significant technical challenge. We have developed two strategies that allow for controlled attachment of a variety of proteins on viral particles using covalent and noncovalent principles. In one strategy, an interaction between domain 4 of anthrax protective antigen and its receptor was used to display multiple copies of a target protein on virus-like particles. In the other, expressed protein ligation and aniline-catalyzed oximation was used to display covalently a model protein. The latter strategy, in particular, yielded nanoparticles that induced potent immune responses to the coupled protein, suggesting potential applications in vaccine development.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
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Vírion
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Proteínas Recombinantes
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Nanotecnologia
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Nanoestruturas
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Proteínas Ligantes de Maltose
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Antígenos de Bactérias
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article