Electrochemically-driven large amplitude pH cycling for acid-base driven DNA denaturation and renaturation.
Anal Chem
; 83(12): 4930-5, 2011 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-21561110
ABSTRACT
In this paper, we present an electrochemically driven large amplitude pH alteration method based on a serial electrolytic cell involving a hydrogen permeable bifacial working electrode such as Pd thin foil. The method allows solution pH to be changed periodically up to ±4~5 units without additional alteration of concentration and/or composition of the system. Application to the acid-base driven cyclic denaturation and renaturation of 290 bp DNA fragments is successfully demonstrated with in situ real-time UV spectroscopic characterization. Electrophoretic analysis confirms that the denaturation and renaturation processes are reversible without degradation of the DNA. The serial electrolytic cell based electrochemical pH alteration method presented in this work would promote investigations of a wide variety of potential-dependent processes and techniques.
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1
Base de dados:
MEDLINE
Assunto principal:
Ácidos
/
DNA
/
Técnicas Eletroquímicas
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article