Isolation and characterization of 12 microsatellite loci for cross-species amplification in the cyprinid gudgeons Squalidus chankaensis and S. japonicus.

ABSTRACT

Twelve dinucleotide markers were successfully isolated and characterized from a microsatellite-enriched genomic library obtained for the gudgeon Squalidus chankaensis biwae. These markers were also available for the congeners S. c. tsuchigae and S. japonicus from Japan, which had five to 46 alleles and an expected heterozygosity ranging from zero to 0.946. Linkage equilibrium was observed at all loci, and most loci did not show significant deviation from Hardy-Weinberg equilibrium. The isolated microsatellite markers will be useful for genetic diversity studies of Squalidus populations.