Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi.
J Microbiol Methods
; 86(2): 243-7, 2011 Aug.
Article
em En
| MEDLINE
| ID: mdl-21605603
ABSTRACT
Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Reação em Cadeia da Polimerase
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Borrelia burgdorferi
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Biologia Molecular
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article