Immunomodulatory effects of Lactobacillus casei administration in a mouse model of gliadin-sensitive enteropathy.
Scand J Immunol
; 74(4): 335-41, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21615450
ABSTRACT
Coeliac disease (CD) is a very common food-sensitive enteropathy, which is triggered by gluten ingestion and is mediated by CD4(+) T cells. In addition, alterations in the intestinal microbiota that is normally involved in the homeostasis of GALT (gut-associated lymphoid tissue) seem to play a role in CD. In accordance with these findings, we previously reported that Lactobacillus casei can induce a strong enhancement of the T cell-mediated response to gliadin without inducing enteropathy. In this study, we analysed the effects of L. casei administration in a mouse model of gliadin-induced villous damage that was recently developed and involves the inhibition of cyclo-oxygenase (COX) activities in gliadin-sensitized HLA-DQ8 transgenic mice. To address the issue, we assessed the weight loss, the intestinal cytokine pattern, the density of CD25(+) cells and morphometry of the gut mucosa. We confirmed that COX inhibition in sensitized mice caused villus blunting, dysregulated expression of tumour necrosis factor (TNF)-α and reduced gliadin-specific IL-2 production. Notably, the administration of probiotic strain induced a complete recovery of villus blunting. This finding was associated with a delay in weight decrease and a recovery of basal TNF-α levels, whereas the numbers of CD25(+) cells and the levels of IL-2 remained unchanged. In conclusion, our data suggest that the administration of L. casei can be effective in rescuing the normal mucosal architecture and GALT homeostasis in a mouse model of gliadin-induced enteropathy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Antígenos HLA-DQ
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Doença Celíaca
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Prostaglandina-Endoperóxido Sintases
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Gliadina
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Lacticaseibacillus casei
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article