[Preparing of a ssDNA target in a single-round PCR with low-melt excess primer for hybridization with microarrays].
Mol Biol (Mosk)
; 45(2): 267-71, 2011.
Article
em Ru
| MEDLINE
| ID: mdl-21634114
ABSTRACT
Method of ssDNA preparing in single-round PCR for microarray application is described. The approach is exemplified on genotyping of DARC gene. It is opposed to two-round PCR that consists of separate symmethric and asymmethric stages. Implementation of reaction in single round is achieved by means of low-melt excess internal primer application. The primer do not anneal during symmethric stage but after decreasing of annealing temperature on asymmethric stage. The results indicate effective oligonucleotide microarray genotyping. The approach reduces time requirements and risk of contamination.
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Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Reação em Cadeia da Polimerase
/
Análise de Sequência com Séries de Oligonucleotídeos
Idioma:
Ru
Ano de publicação:
2011
Tipo de documento:
Article