Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule.
Proc Natl Acad Sci U S A
; 108(27): 11199-204, 2011 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-21690402
ABSTRACT
Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4(+) T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4(+) T cells and plasma implies ongoing exchange between these compartments both early and late after infection.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Linfócitos T CD4-Positivos
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Infecções por HIV
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HIV-1
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article