A strategic and systematic approach for the determination of biosensor regeneration conditions.
J Immunol Methods
; 371(1-2): 165-9, 2011 Aug 31.
Article
em En
| MEDLINE
| ID: mdl-21703275
ABSTRACT
Determining the optimal conditions for surface regeneration is fundamental for performance of efficient and robust protein-protein interaction kinetic studies. We devised a systematic methodology comprised of an automated seven-cycle analyte and buffer injection Biacore scheme and data interpretation algorithm. The efficiency and utility is illustrated using an antigen/monoclonal antibody interaction that required ultimately six pulses of acid for regeneration. This technique has broad applicability to any biosensor assay that requires regeneration of a surface.
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1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article