Inhibition of pre-B cell colony-enhancing factor attenuates inflammation and apoptosis induced by pandemic H1N1 2009 in lung endothelium.
Respir Physiol Neurobiol
; 178(2): 235-41, 2011 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-21726671
ABSTRACT
The recent pandemic influenza A (H1N1 2009) virus infection has caused acute lung injury in susceptible population resulting in high mortality in ICU patients. In this report, we observed the effect of pre-B cell colony-enhancing factor (PBEF) on the inflammation and apoptosis in H1N1-infected human pulmonary microvascular endothelial cells (HPMECs). We constructed an in vitro HPMEC monolayer model. The results showed that H1N1 2009 induced the increased expression of inflammatory cytokines (IL-6/IL-8/TNF-α/IP-10) and apoptosis factors (FasL/TRAIL) in infected HPMECs. However, PBEF silencing with siRNA inhibited the expression of some inflammatory cytokines and decreased the apoptosis mediated by FasL. We conclude that PBEF might be partially responsible for the localized inflammatory response to H1N1 2009 in the lung microvascular endothelium and the H1N1-induced endothelial cell apoptosis probably through the FasL-mediated pathway.
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Base de dados:
MEDLINE
Assunto principal:
Apoptose
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Mediadores da Inflamação
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Mucosa Respiratória
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Proteínas Reguladoras de Apoptose
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Influenza Humana
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Vírus da Influenza A Subtipo H1N1
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Nicotinamida Fosforribosiltransferase
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Pandemias
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article