Magnetic/polymer/nanogold complex using as a novel enzyme support.
J Nanosci Nanotechnol
; 11(6): 5223-7, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21770168
ABSTRACT
A novel enzyme support based on magnetic/polymer/nanogold complex was prepared in this study. At first, carboxymethyl chitosan (CMC) were connected onto the surface of Fe3O4 nanoparticles, and then gold nanoparticles were in situ synthesized and bound to CMC chains, resulting in Fe3O4/CMC/nanogold complex. The complex was determined by UV-vis spectroscopy, transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Horseradish peroxidase (HRP) was immobilized on the Fe3O4/CMC/nanogold complex. Compared with free HRP, the immobilized HRP exhibits higher catalytic activity and better storage stability. This Fe3O4/CMC/nanogold complex can serve as an excellent support for enzymes.
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Base de dados:
MEDLINE
Assunto principal:
Quitosana
/
Enzimas Imobilizadas
/
Nanopartículas de Magnetita
/
Ouro
/
Peroxidase do Rábano Silvestre
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article