Putrescine is required for the expression of eif-5a in Trichomonas vaginalis.
Mol Biochem Parasitol
; 180(1): 8-16, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21801756
ABSTRACT
Recently, we found that Trichomonas vaginalis contains a eukaryotic translation initiation factor 5A (TveIF-5A) with unknown function in this parasite. eIF-5A is the only cellular protein dependent of polyamines to form a hypusine residue, an unusual basic amino acid that is post-translationally formed by modification of a single specific lysine residue in an eIF-5A precursor protein. The purpose of this study was to determine the effect of a putrescine analogue, 1,4-diamino-2-butanone (DAB), on tveif-5a mRNA and TveIF-5A protein expression. TveIF-5A protein expression was reduced by inhibition of putrescine biosynthesis, and tveif-5a mRNA levels were reduced â¼90%, as shown by western blot and immunofluorescence assays. Cycloheximide treatment reduced the amount of mature TveIF-5A protein at 4h and decreased the tveif-5a transcript level at 2h, according to western blot, RT-PCR and qRT-PCR analyses. Actinomycin D treatment showed that the tveif-5a mRNA had half-life of â¼2.5h in DAB-treated parasites. The half-life of tveif-5a mRNA was â¼4.5h under exogenous putrescine conditions. These results suggest that putrescine is required for tveif-5a mRNA stability, and it is necessary for the expression, stability and maturation of TveIF-5A protein.
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Base de dados:
MEDLINE
Assunto principal:
Trichomonas vaginalis
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Putrescina
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Proteínas de Protozoários
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Fatores de Iniciação de Peptídeos
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Regulação da Expressão Gênica
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Proteínas de Ligação a RNA
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article