Xeno-free culture of human pluripotent stem cells.
Methods Mol Biol
; 767: 125-36, 2011.
Article
em En
| MEDLINE
| ID: mdl-21822871
ABSTRACT
Stem cell culture systems that rely on undefined animal-derived components introduce variability to the cultures and complicate their therapeutic use. The derivation of human embryonic stem cells and the development of methods to produce induced pluripotent stem cells combined with their potential to treat human diseases have accelerated the drive to develop xenogenic-free, chemically defined culture systems that support pluripotent self-renewal and directed differentiation. In this chapter, we describe four xeno-free culture systems that have been successful in supporting undifferentiated growth of hPSCs as well as methods for xeno-free subculture and cryopreservation of hPSCs. Each culture system consists of a xeno-free growth medium and xeno-free substratum (1) TeSR2™ with human recombinant laminin (LN-511); (2) NutriStem™ with LN-511; (3) RegES™ with human foreskin fibroblasts (hFFs); (4) KO-SR Xeno-Free™/GF cocktail with CELLstart™ matrix.
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1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
/
Células-Tronco Pluripotentes
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article