[The affect of Si-Rac1b on the malignant biological behaviors of colorectal cancer cell].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 42(4): 451-4, 460, 2011 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-21866624
ABSTRACT
OBJECTIVE:
To observe the affect of Small interfering RNA Rac1b (Si-Rac1b) on the malignant biological behaviors of colorectal cancer cell including the proliferation, migration, invasion and apoptosis of the cells.METHODS:
Mediated by lipofectamine 2000, Si-Rac1b was transfected into colorectal cancer cell line SW1116 (with overexpression of Rac1b). The expression of Rac1b was detected by Western blotting and RT-PCR. The CCK-8 assay was used to analyze the cell proliferation, the Wound-healing assay and invasion assay were respectively applied to analyze the cell migration and invasion, and the Hoechst 33258 was used to evaluate the apoptotic index.RESULTS:
Si-Rac1b can knock down the Rac1b but not Rac1 both in the level of mRNA and protein. In addition, Si-Rac1b could singnificantly facilitate the cell proliferation, migration, invasion and control the cell apoptosis.CONCLUSION:
Si-Rac1b could partically reverse the malignant phenotypes of colorectal cancer cell.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
/
Apoptose
/
Proteínas rac1 de Ligação ao GTP
/
RNA Interferente Pequeno
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article