Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels.
Nat Mater
; 10(10): 799-806, 2011 Oct.
Article
em En
| MEDLINE
| ID: mdl-21874004
ABSTRACT
Three-dimensional (3D) protein-patterned scaffolds provide a more biomimetic environment for cell culture than traditional two-dimensional surfaces, but simultaneous 3D protein patterning has proved difficult. We developed a method to spatially control the immobilization of different growth factors in distinct volumes in 3D hydrogels, and to specifically guide differentiation of stem/progenitor cells therein. Stem-cell differentiation factors sonic hedgehog (SHH) and ciliary neurotrophic factor (CNTF) were simultaneously immobilized using orthogonal physical binding pairs, barnase-barstar and streptavidin-biotin, respectively. Barnase and streptavidin were sequentially immobilized using two-photon chemistry for subsequent concurrent complexation with fusion proteins barstar-SHH and biotin-CNTF, resulting in bioactive 3D patterned hydrogels. The technique should be broadly applicable to the patterning of a wide range of proteins.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Hidrogéis
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Peptídeos e Proteínas de Sinalização Intercelular
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Materiais Biomiméticos
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Alicerces Teciduais
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article