A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides.
Curr Protoc Cell Biol
; Chapter 26: Unit26.13, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21898337
ABSTRACT
Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
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Base de dados:
MEDLINE
Assunto principal:
Fármacos Anti-HIV
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Células Epiteliais
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Ensaios de Triagem em Larga Escala
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Genitália Feminina
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Modelos Biológicos
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article