Evolution and protein packaging of small-molecule RNA aptamers.
ACS Nano
; 5(10): 7722-9, 2011 Oct 25.
Article
em En
| MEDLINE
| ID: mdl-21899290
ABSTRACT
A high-affinity RNA aptamer (K(d) = 50 nM) was efficiently identified by SELEX against a heteroaryldihydropyrimidine structure, chosen as a representative drug-like molecule with no cross reactivity with mammalian or bacterial cells. This aptamer, its weaker-binding variants, and a known aptamer against theophylline were each embedded in a longer RNA sequence that was encapsidated inside a virus-like particle by a convenient expression technique. These nucleoprotein particles were shown by backscattering interferometry to bind to the small-molecule ligands with affinities similar to those of the free (nonencapsidated) aptamers. The system therefore comprises a general approach to the production and sequestration of functional RNA molecules, characterized by a convenient label-free analytical technique.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Capsídeo
/
Aptâmeros de Nucleotídeos
/
Técnica de Seleção de Aptâmeros
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article