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[Association between the change regularity of peripheral blood mononuclear cell mitochondrial deoxyribonucleic acid content and human immunodeficiency virus-related lipodystrophy].
Wu, Peng; Gu, Wei-bin; Zhang, Lu; Li, Yan-ling; Qiu, Zhi-feng; Han, Yang; Xie, Jing; Li, Tai-sheng.
Afiliação
  • Wu P; Department of Infectious Diseases, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi ; 91(21): 1448-52, 2011 Jun 07.
Article em Zh | MEDLINE | ID: mdl-21914278
ABSTRACT

OBJECTIVE:

To investigate the change regularity of peripheral blood mononuclear cell (PBMC) mtDNA (mitochondrial deoxyribonucleic acid) content and its association with HIV-LD (human immunodeficiency virus-related lipodystrophy) in HAART (highly active antiretroviral therapy).

METHODS:

At baseline, Months 6 and 24 of therapy, the cryopreserved PBMC were collected from 33 patients on a regular follow-up at our clinic. Among them, 17 had HIV-LD. Then total DNA was extracted and mtDNA content quantified by real-time PCR (polymerase chain reaction).

RESULTS:

The HIV/AIDS patients had a lower content of PBMC mtDNA (2(-ΔΔCt)) than the healthy controls at baseline (9.578 vs 17.195, P < 0.01). The mtDNA content was lower in the HIV-LD group than that in the no LD (NLD) group at each time point of therapy (13.619 vs 5.775, 6.360 vs 1.387, 7.170 vs 1.266, all P < 0.05). In the HIV-LD group, the half- and 2-year PBMC mtDNA content was markedly lower than those at baseline (both P < 0.05). And the change of mtDNA content (within half a year) was earlier than the onset of clinical HIV-LD at one year later. In the NLD group, the PBMC mtDNA content have an insignificant change after therapy. The mtDNA content decreased significantly in stavudine (d4T)-containing regimen group after treatment (P < 0.01), but showed no significant change in zidovudine (AZT)-containing regimen group after therapy.

CONCLUSION:

The decreased content of PBMC mtDNA after HIV infection and during HAART therapy is associated with HIV-LD. Nucleoside reverse transcriptase inhibitor, especially d4T, plays an important role in the progression of HIV-LD.
Assuntos
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Base de dados: MEDLINE Assunto principal: DNA Mitocondrial / Leucócitos Mononucleares / Síndrome de Lipodistrofia Associada ao HIV Idioma: Zh Ano de publicação: 2011 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: DNA Mitocondrial / Leucócitos Mononucleares / Síndrome de Lipodistrofia Associada ao HIV Idioma: Zh Ano de publicação: 2011 Tipo de documento: Article