Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway.
Anal Biochem
; 419(2): 271-6, 2011 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-21925479
ABSTRACT
Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 µg of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry.
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1
Base de dados:
MEDLINE
Assunto principal:
Bioquímica
/
Clorofilídeos
/
Vias Biossintéticas
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article