Toward an understanding of the protein interaction network of the human liver.
Mol Syst Biol
; 7: 536, 2011 10 11.
Article
em En
| MEDLINE
| ID: mdl-21988832
ABSTRACT
Proteome-scale protein interaction maps are available for many organisms, ranging from bacteria, yeast, worms and flies to humans. These maps provide substantial new insights into systems biology, disease research and drug discovery. However, only a small fraction of the total number of human protein-protein interactions has been identified. In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins. The data set has a validation rate of over 72% as determined by three independent biochemical or cellular assays. The network includes metabolic enzymes and liver-specific, liver-phenotype and liver-disease proteins that are individually critical for the maintenance of liver functions. The liver enriched proteins had significantly different topological properties and increased our understanding of the functional relationships among proteins in a liver-specific manner. Our data represent the first comprehensive description of a HLPN, which could be a valuable tool for understanding the functioning of the protein interaction network of the human liver.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteoma
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Mapeamento de Interação de Proteínas
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Proteômica
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Biologia de Sistemas
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Mapas de Interação de Proteínas
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Fígado
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article