[The effect of RhoE on CD44 promoter and the malignant behaviors of colorectal cancer cell].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 42(5): 589-93, 2011 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-22007478
ABSTRACT
OBJECTIVE:
To study the effect of RhoE on the transcriptional regulation of cd44 and in vivo tumorigenicity of nude mice.METHODS:
cd44 promoter was amplified from human embryonic kidney HEK293 cells with PCR and insert into Dual-Luciferase Reporter plasmid pGL3-Basic. After confirmed with sequence analysis, the generated recombinant was transfected into SW480 and LoVo cells to monitor their activity. Colon cancer SW480 and LoVo cells were cotransfected with pGL3-CD44 promoter along with pcDNA3. 1-RhoE and pcDNA3. 1 respectively. SW480 and LoVo cells were stably transfected with pcDNA3. 1-RhoE and the control group and were inoculated into nude mice to observe tumor growth. Immunohistochemistry assay was applied to observe the morphology of tumor cells and the expression of CD44 molecules.RESULTS:
The cd44 promoter sequence was amplified correctly, Dual-Luciferase Reporter Assay showed that the constructed reporter gene has promoter activity. The expression of cd44 promoter sequence containing reporter gene in pcDNA3. 1-RhoE expression positive LoVo cells was inhibited; HE staining demonstrated that the pcDNA3. 1-RhoE transfected tumor cells was significantly smaller than that in the control group, and consistent size and shape tumor cells were observed but no tumor giant cells, the corresponding volume of the tumor nuclei were also small.CONCLUSION:
RhoE could partially reverse the malignant biological behavior of tumors by inhibiting the transcriptional regulation of cd44 promoter.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
/
Genes Supressores de Tumor
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Regiões Promotoras Genéticas
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Receptores de Hialuronatos
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Proteínas rho de Ligação ao GTP
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article