Improvement of peak shape in aqueous normal phase analysis of anionic metabolites.
J Sep Sci
; 34(24): 3509-16, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-22009714
ABSTRACT
The problem of poor peak shape for multiply charged negative-ion analytes under aqueous normal phase (ANP) conditions is investigated. Because less than adequate efficiency and symmetry can occur with a variety of mobile phases, gradients and additives, and to varying degrees depending on the instrument, sources other than solute/stationary phase interactions are more likely the cause. Since it is known that many of these compounds can interact strongly with metal ions, addition of a chelating agent to the mobile phase and/or the sample solvent was tested. In particular, ethylenediaminetetraacetic acid (EDTA) is a compound that forms strong complexes with most di-and tri-valent metal ions and can be used to verify whether trace amounts of these species are the source of the problem. In addition, the retention of a number of anionic compounds was measured at various concentrations of ammonium acetate and formate with EDTA in the mobile phase.
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Base de dados:
MEDLINE
Assunto principal:
Cobre
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Ferro
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article