Engineered cell homing.
Blood
; 118(25): e184-91, 2011 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-22034631
ABSTRACT
One of the greatest challenges in cell therapy is to minimally invasively deliver a large quantity of viable cells to a tissue of interest with high engraftment efficiency. Low and inefficient homing of systemically delivered mesenchymal stem cells (MSCs), for example, is thought to be a major limitation of existing MSC-based therapeutic approaches, caused predominantly by inadequate expression of cell surface adhesion receptors. Using a platform approach that preserves the MSC phenotype and does not require genetic manipulation, we modified the surface of MSCs with a nanometer-scale polymer construct containing sialyl Lewis(x) (sLe(x)) that is found on the surface of leukocytes and mediates cell rolling within inflamed tissue. The sLe(x) engineered MSCs exhibited a robust rolling response on inflamed endothelium in vivo and homed to inflamed tissue with higher efficiency compared with native MSCs. The modular approach described herein offers a simple method to potentially target any cell type to specific tissues via the circulation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Transplante de Células-Tronco Mesenquimais
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article