Enrichment of breast cancer stem cells using a keratinocyte serum-free medium.
Chin Med J (Engl)
; 124(18): 2934-6, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-22040505
ABSTRACT
BACKGROUND:
Keratinocyte serum-free medium (K-SFM) is a defined medium used to support the growth of primary keratinocytes and embryonic stem cell. The aim of this research was to optimize enrichment of breast cancer stem cells (CSCs) using K-SFM.METHODS:
A K-SFM was used to enrich CSCs from two breast cancer cell lines and a primary culture of breast cancer. RPMI-1640 supplemented with 10% fetal calf serum (FCS) was used as a control. CSCs were identified with flow cytometry using CD44(+)/CD24(-) as molecular markers. The expression of a variety of CSC markers (Oct-4, ABCG2, Nanog, N-cadherin, and E-cadherin) was analyzed with real-time PCR.RESULTS:
Much higher percentage of CSCs was achieved with K-SFM 17.3% for MCF-7 cells, 17.4% for SKBR-3, and 20.0% for primary breast cancer culture. Less than 1% CSC was achieved using RPMI-1640 supplemented with 10% FCS. In comparison to the CSCs obtained with RPMI-1640, CSCs in the K-SFM expressed higher levels of Oct-4, ABCG2, Nanog and N-cadherin, and lower level of E-cadherin.CONCLUSION:
K-SFM is an optimal culture medium to maintain and to enrich breast CSCs.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Neoplásicas
/
Queratinócitos
/
Meios de Cultura Livres de Soro
/
Técnicas de Cultura de Células
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article