A two-stage algorithm for Clostridium difficile including PCR: can we replace the toxin EIA?
J Hosp Infect
; 80(1): 82-4, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22104474
ABSTRACT
A two step, three-test algorithm for Clostridium difficile infection (CDI) was reviewed. Stool samples were tested by enzyme immunoassays for C. difficile common antigen glutamate dehydrogenase (G) and toxin A/B (T). Samples with discordant results were tested by polymerase chain reaction detecting the toxin B gene (P). The algorithm quickly identified patients with detectable toxin A/B, whereas a large group of patients excreting toxigenic C. difficile but with toxin A/B production below detection level (G(+)T(-)P(+)) was identified separately. The average white blood cell count in patients with a G(+)T(+) result was higher than in those with a G(+)T(-)P(+) result.
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Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Reação em Cadeia da Polimerase
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Clostridioides difficile
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Infecções por Clostridium
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Técnicas de Laboratório Clínico
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article