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Constitutive stimulation of vascular smooth muscle cells by angiotensin II derived from an adenovirus encoding a furin-cleavable fusion protein.
Takayanagi, Takehiko; Bourne, Allison M; Kimura, Keita; Takaguri, Akira; Elliott, Katherine J; Eguchi, Kunie; Eguchi, Satoru.
Afiliação
  • Takayanagi T; Cardiovascular Research Center, Department of Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania, USA.
Am J Hypertens ; 25(3): 280-3, 2012 Mar.
Article em En | MEDLINE | ID: mdl-22113169
BACKGROUND: To fill the gap between acute and chronic stimulation methods of angiotensin II (Ang II) and obtain relevant signaling information, we have made an adenovirus vector encoding a furin-cleavable Ang II fusion protein. METHODS: Vascular smooth muscle cells (VSMCs) were infected with adenovirus to evaluate Ang II production. Also, expression of early growth response-1 (Egr-1) and hypertrophic responses were examined in VSMCs. RESULTS: Acute stimulation of VSMCs with synthetic Ang II showed the peptide had a half-life of less than 1 h. Infection of VSMCs with Ang II adenovirus showed a time-dependent production of Ang II as early as 2 days and up to 7 days postinfection. The Ang II adenovirus induced VSMC hypertrophy, stimulated Egr-1 expression, and suppressed Ang II type 1 receptor mRNA expression. Chronic Ang II infusion in mice for 2 weeks markedly enhanced Egr-1 immunostaining in carotid artery compared with the control saline infusion. CONCLUSION: Application of the Ang II adenovirus vector to cultured cells will be useful to elucidate molecular and signaling mechanisms of cardiovascular diseases associated with enhanced Ang II production.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Angiotensina II / Adenoviridae / Artéria Carótida Primitiva / Proteína 1 de Resposta de Crescimento Precoce / Vetores Genéticos / Músculo Liso Vascular Idioma: En Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Angiotensina II / Adenoviridae / Artéria Carótida Primitiva / Proteína 1 de Resposta de Crescimento Precoce / Vetores Genéticos / Músculo Liso Vascular Idioma: En Ano de publicação: 2012 Tipo de documento: Article