Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.
Nat Biotechnol
; 29(12): 1117-9, 2011 Nov 27.
Article
em En
| MEDLINE
| ID: mdl-22119740
ABSTRACT
We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.
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Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
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Diferenciação Celular
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Linhagem da Célula
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Metilação de DNA
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Epigênese Genética
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Células-Tronco Pluripotentes Induzidas
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Sangue Fetal
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article