Identification of a novel isoform of DHRS4 protein with a nuclear localization signal.
Gene
; 494(2): 161-7, 2012 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-22227495
ABSTRACT
The DHRS4 gene encodes an NADP(H)-dependent retinol dehydrogenase/reductase (NRDR) and plays an important role in regulating the synthesis of retinoic acid. In the present study, we identified a novel splice RNA variant, designated NRDRA2, of the human DHRS4 gene by RT-PCR, 3' RACE, and 5' RACE. NRDRA2 mRNA lacked exons 4 and 6, and had a shift in the reading frame when compared to DHRS4 mRNA, resulting in loss of the peroxisomal targeting signal of NRDR and gain of a nuclear localization signal in the predicted NRDRA2 protein. Endogenous NRDRA2 protein was identified in the human cervical carcinoma cell line HeLa by immunoprecipitation and mass spectrometric assay. A green fluorescent protein reporter assay showed that NRDRA2 protein mainly localized to the nuclei, confirming the sequence at its C-terminus as a legitimate nuclear localization signal sequence. This study identifies the alternative transcript variant NRDRA2 encoding a subcellular nuclear localized NRDRA2 protein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Sinais de Localização Nuclear
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Isoformas de Proteínas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article