[mTOR/p70s6k signaling pathway is involved in megakaryocyte polyploidization].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 28(1): 21-4, 2012 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-22230498
ABSTRACT
AIM:
To investigate the mechanisms of megakaryocyte polyploid cell cycle control.METHODS:
The expression and phosphorylation of mTOR/p70s6k pathway proteins was detected by western blot. Double-labeling techniques were used to investigate in which of the phase of the polyploid cell cycle S6K1 at Thr421/Ser424 are phosphorylated.RESULTS:
Nocodazole induced a relatively synchronized polyploidization in Dami cells. The expression of mTOR and the phosphorylation of mTOR at Ser2448 increased when Dami cells begin to progress from G1 to S-phase in cell cycle. Analysis of flow cytometry showed that phosphorylation of S6K1 at Thr421/Ser424 increased at G2/M-phase.CONCLUSION:
mTOR/S6K1 pathway is involved in megakaryocyte polyploid cell cycle control.
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Base de dados:
MEDLINE
Assunto principal:
Poliploidia
/
Megacariócitos
/
Transdução de Sinais
/
Proteínas Quinases S6 Ribossômicas 70-kDa
/
Serina-Treonina Quinases TOR
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article