Developmental changes in the localization of protein kinase CK2 in non-diapause and diapause eggs of the silkworm, Bombyx mori.
Zoolog Sci
; 29(1): 6-10, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22233490
ABSTRACT
To analyze the role of protein kinase CK2 (CK2) during early embryogenesis in non-diapause and diapause of the silkworm, the distribution and localization of Bombyx mori CK2 (BmCK2) were investigated by an immunohistochemical technique using antibodies against the α- and ß-subunits of BmCK2. Both were localized in blastoderm cells of non-diapause and diapause eggs until 24 h after oviposition. More than 24 h after oviposition, however, the distribution of BmCK2 was different in non-diapause and diapause eggs. In non-diapause eggs, BmCK2 was mainly localized in yolk cells. In contrast, in diapause eggs, the localization was mainly observed in germ-band cells. Furthermore, we confirmed that the RNA helicase-like protein that was localized together with BmCK2 in non-diapause eggs was phosphorylated by BmCK2 in vitro. These data suggest that the role of BmCK2 is different in non-diapause and diapause eggs.
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Base de dados:
MEDLINE
Assunto principal:
Óvulo
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Bombyx
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Regulação Enzimológica da Expressão Gênica
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Regulação da Expressão Gênica no Desenvolvimento
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Transporte Proteico
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Caseína Quinase II
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article