Coordinate-bond-dependent solid-phase organic synthesis of biotinylated desferrioxamine B: a new route for metal-specific probes.
Chem Commun (Camb)
; 48(14): 2003-5, 2012 Feb 14.
Article
em En
| MEDLINE
| ID: mdl-22234401
ABSTRACT
Desferrioxamine B (DFOB) was biotinylated at the pendant amine using solid-phase organic synthesis (SPOS) on a matrix used conventionally for metal affinity chromatography. The strength of the DFOB-matrix coordinate bonds was functionally equivalent to a covalent bond which underpinned the veracity of the SPOS format. After washing excess reagents, biotin-DFOB was eluted from the matrix with water at pH 6.
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1
Base de dados:
MEDLINE
Assunto principal:
Desferroxamina
/
Metais
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article