Normal-phase liquid chromatography coupled with electrospray ionization mass spectrometry for chiral separation and quantification of clevudine and its enantiomer in human plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
; 885-886: 97-102, 2012 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-22265667
ABSTRACT
A new, simple and enantioselective normal-phase liquid chromatography-mass spectrometry method was presented for the quantification of clevudine and its enantiomer in human plasma. A C18 cartridge was used in this method to extract the enantiomers in 200µL plasma followed by a chiral separation on a cellulose-based LC column with mobile phase consisted of hexane, methanol and ethanol (622810, V/V/V). The eluate was directed to a mass spectrometry through an electrospray ionization interface. A transition of m/z 261.0 to m/z 126.8 was used for monitoring of clevudine and its enantiomer. This method showed good linearity (R>0.997), precision (<9.6%) and accuracy (within 95.48-105.9%) within a range of 10-1000ng/mL for the enantiomers and has been applied to the pharmacokinetics study of clevudine capsules in human plasma.
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1
Base de dados:
MEDLINE
Assunto principal:
Arabinofuranosiluracila
/
Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas por Ionização por Electrospray
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article