Polygalasaponin F induces long-term potentiation in adult rat hippocampus via NMDA receptor activation.
Acta Pharmacol Sin
; 33(4): 431-7, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22286914
AIM: To investigate the effect and underlying mechanisms of polygalasaponin F (PGSF), a triterpenoid saponin isolated from Polygala japonica, on long-term potentiation (LTP) in hippocampus dentate gyrus (DG) of anesthetized rats. METHODS: Population spike (PS) of hippocampal DG was recorded in anesthetized male Wistar rats. PGSF, the NMDAR inhibitor MK801 and the CaMKII inhibitor KN93 were intracerebroventricularly administered. Western blotting analysis was used to examine the phosphorylation expressions of NMDA receptor subunit 2B (NR2B), Ca(2+)/calmodulin-dependent kinase II (CaMKII), extracellular signal-regulated kinase (ERK), and cAMP response element-binding protein (CREB). RESULTS: Intracerebroventricular administration of PGSF (1 and 10 µmol/L) produced long-lasting increase of PS amplitude in hippocampal DG in a dose-dependent manner. Pre-injection of MK801 (100 µmol/L) or KN93 (100 µmol/L) completely blocked PGSF-induced LTP. Furthermore, the phosphorylation of NR2B, CaMKII, ERK, and CREB in hippocampus was significantly increased 5-60 min after LTP induction. The up-regulation of p-CaMKII expression could be completely abolished by pre-injection of MK801. The up-regulation of p-ERK and p-CREB expressions could be partially blocked by pre-injection of KN93. CONCLUSION: PGSF could induce LTP in hippocampal DG in anesthetized rats via NMDAR activation mediated by CaMKII, ERK and CREB signaling pathway.
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Base de dados:
MEDLINE
Assunto principal:
Saponinas
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Triterpenos
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Receptores de N-Metil-D-Aspartato
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Potenciação de Longa Duração
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Giro Denteado
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article