Imaging the glycome in living systems.
Methods Enzymol
; 505: 401-19, 2012.
Article
em En
| MEDLINE
| ID: mdl-22289465
ABSTRACT
The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Microscopia Confocal
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Rastreamento de Células
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Química Click
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article