Signal amplification by enzymatic reaction in an immunosensor based on localized surface plasmon resonance (LSPR).
Sensors (Basel)
; 10(3): 2045-53, 2010.
Article
em En
| MEDLINE
| ID: mdl-22294913
ABSTRACT
An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-γ (IFN-γ) capture antibody was immobilized on the gold NIs, followed by binding of IFN-γ to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Imunoenzimáticas
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Ressonância de Plasmônio de Superfície
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Nanotecnologia
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Nanoestruturas
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article