Mono-allelic retrotransposon insertion addresses epigenetic transcriptional repression in human genome.
J Biomed Sci
; 19: 13, 2012 Feb 02.
Article
em En
| MEDLINE
| ID: mdl-22300442
ABSTRACT
BACKGROUND:
Retrotransposons have been extensively studied in plants and animals and have been shown to have an impact on human genome dynamics and evolution. Their ability to move within genomes gives retrotransposons to affect genome instability.METHODS:
we examined the polymorphic inserted AluYa5, evolutionary young Alu, in the progesterone receptor gene to determine the effects of Alu insertion on molecular environment. We used mono-allelic inserted cell lines which carry both Alu-present and Alu-absent alleles. To determine the epigenetic change and gene expression, we performed restriction enzyme digestion, Pyrosequencing, and Chromatin Immunoprecipitation.RESULTS:
We observed that the polymorphic insertion of evolutionally young Alu causes increasing levels of DNA methylation in the surrounding genomic area and generates inactive histone tail modifications. Consequently the Alu insertion deleteriously inactivates the neighboring gene expression.CONCLUSION:
The mono-allelic Alu insertion cell line clearly showed that polymorphic inserted repetitive elements cause the inactivation of neighboring gene expression, bringing aberrant epigenetic changes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Genoma Humano
/
Metilação de DNA
/
Elementos Alu
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article