Serum from calorie-restricted rats activates vascular cell eNOS through enhanced insulin signaling mediated by adiponectin.
PLoS One
; 7(2): e31155, 2012.
Article
em En
| MEDLINE
| ID: mdl-22319612
ABSTRACT
eNOS activation resulting in mitochondrial biogenesis is believed to play a central role in life span extension promoted by calorie restriction (CR). We investigated the mechanism of this activation by treating vascular cells with serum from CR rats and found increased Akt and eNOS phosphorylation, in addition to enhanced nitrite release. Inhibiting Akt phosphorylation or immunoprecipitating adiponectin (found in high quantities in CR serum) completely prevented the increment in nitrite release and eNOS activation. Overall, we demonstrate that adiponectin in the serum from CR animals increases NO⢠signaling by activating the insulin pathway. These results suggest this hormone may be a determinant regulator of the beneficial effects of CR.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Restrição Calórica
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Adiponectina
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Óxido Nítrico Sintase Tipo III
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Insulina
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article