[Effect of diubiquitin gene silencing by small interfering RNA on proliferation and invasion of tongue carcinoma Tca8113 cells].

ABSTRACT

OBJECTIVE: To study the effect of diubiquitin (FAT10) down-regulation by small interfering RNA-mediated RNA interference (RNAi) on the biological features of tongue carcinoma cell line Tca8113. METHODS: Tca8113 cells were transfected with synthetic small interfering RNA (siRNA) targeting FAT10. Expression of FAT10 mRNA and protein were respectively measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, transfection efficiencies were monitored. The distribution of cell cycle phases was determined using flow cytometry. The proliferative and invasive ability of Tca8113 cells in vitro was evaluated by the colony-forming unit assay and Transwell migration assay respectively. RESULTS: Both FAT10 mRNA and protein expression were significantly decreased in the experimental group (pU-FAT10-siRNA mRAN 0.36 ± 0.03, Protein 0.39 ± 0.04) compared with controls ( CONTROL mRNA 0.95 ± 0.05, Protein 0.69 ± 0.05; pU-siRNA mRNA 0.92 ± 0.07, Protein 0.64 ± 0.05) (P < 0.05). The cell cycle was arrested in the G(1) phase [pU-FAT10-siRNA (72.45 ± 5.81)%, CONTROL (45.95 ± 3.80)%, pU-siRNA (45.95 ± 3.80)%]. The proliferation and invasiveness of treated Tca8113 cells were inhibited in vitro (pU-FAT10-siRNA 41.83 ± 8.19, CONTROL 317.21 ± 69.48, pU-siRNA 339.36 ± 73.84). CONCLUSIONS: Delivery of siRNA targeting FAT10 seems efficient in down-regulating FAT10 expression and diminishing the growth, proliferation and invasiveness of Tca8113 cells, suggesting that siRNA-based strategy targeting FAT10 may lay a foundation for the clinical management of tongue carcinoma.