Species-specific identification of seven vegetable oils based on suspension bead array.
J Agric Food Chem
; 60(9): 2362-7, 2012 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-22324365
ABSTRACT
Species adulteration of vegetable oils has become a main form of adulteration in vegetable oils, severely violating consumer rights and causing disorder in the market. A reliable method of species authentication of vegetable oils is desirable. This paper reports a novel method for identification of seven species of vegetable oils based on suspension bead array. One pair of universal primers and seven species-specific probes were designed targeting rbcl gene of the chloroplast. Each probe was coupled to a unique color-coded microsphere. Biotinylated PCR amplicons of seven oils were hybridized to the complementary probes on microsphere sets. Bound amplicons were detected fluorometrically using a reporter dye, streptavidin-R-phycoeryt hrin (SA-PE). A sample could be analyzed less than 1 h after PCR amplification. With the exception of olive probe, all probes showed no cross-reactivity with other species. Absolute detection limit of the seven probes ranged from 0.01 ng/µL to 0.0001 ng/µL. Detection limit in DNA mixture was from 10% to 5%. Detection of vegetable oils validated the effectiveness of the method. The suspension bead array as a rapid, sensitive, and high-throughput technology has potential to identify more species of vegetable oils with increased species of probes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óleos de Plantas
/
Contaminação de Alimentos
/
DNA de Plantas
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article