Detection and characterization of VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in a clinical isolate of Enterobacter cloacae.
Antimicrob Agents Chemother
; 56(6): 3283-7, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22391550
We report the first description of the metallo-ß-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in Enterobacter cloacae 11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium. bla(VIM-31) was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lower k(cat) (except for ertapenem) and higher K(m) values for VIM-31.
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1
Base de dados:
MEDLINE
Assunto principal:
Beta-Lactamases
/
Enterobacter cloacae
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article