Isolation of insulin-producing cells from different populations of multipotent stromal cells of the umbilical cord and human adipose tissue.
Bull Exp Biol Med
; 151(1): 114-20, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-22442815
ABSTRACT
Stromal cells of adipose tissue and human umbilical cord were isolated by the original method from general populations of multipotent subpopulation of multipotent stromal cells exhibiting perivascular phenotype (CD146+, CD31-). Effective directed differentiation of these cells into insulin-producing cells by transient transfection of the gene Pdx1 was demonstrated. Transfection multipotent stromal cells CD146-, CD31- derived from adipose tissue and umbilical cord and isolated by the standard method, did not result in activation of insulin gene transcription. It was shown that the expression of nestin was not necessary for effective pancreatic cell differentiation.
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Base de dados:
MEDLINE
Assunto principal:
Transativadores
/
Tecido Adiposo
/
Proteínas de Homeodomínio
/
Células Secretoras de Insulina
/
Sangue Fetal
/
Células-Tronco Mesenquimais
/
Insulina
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article