Rational design of DNA sequence-specific zinc fingers.
FEBS Lett
; 586(6): 918-23, 2012 Mar 23.
Article
em En
| MEDLINE
| ID: mdl-22449981
ABSTRACT
We developed a rational scheme for designing DNA binding proteins. The scheme was applied for a zinc finger protein and the designed sequences were experimentally characterized with high DNA sequence specificity. Starting with the backbone of a known finger structure, we initially calculated amino acid sequences compatible with the expected structure and the secondary structures of the designed fingers were then experimentally confirmed. The DNA-binding function was added to the designed finger by reconsidering a section of the amino acid sequence and computationally selecting amino acids to have the lowest protein-DNA interaction energy for the target DNA sequences. Among the designed proteins, one had a gap between the lowest and second lowest protein-DNA interaction energies that was sufficient to give DNA sequence-specificity.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
Sequência de Bases
/
Engenharia de Proteínas
/
Dedos de Zinco
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article