Adeno-associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice.
Curr Protoc Neurosci
; Chapter 1: Unit 1.20.1-18, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22470147
ABSTRACT
Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic-guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia de Proteínas
/
Dependovirus
/
Técnicas de Rastreamento Neuroanatômico
/
Vetores Genéticos
/
Proteínas Luminescentes
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article