Anti-aging effects of hesperidin on Saccharomyces cerevisiae via inhibition of reactive oxygen species and UTH1 gene expression.
Biosci Biotechnol Biochem
; 76(4): 640-5, 2012.
Article
em En
| MEDLINE
| ID: mdl-22484922
ABSTRACT
This study used a replicative lifespan assay of K6001 yeast to screen anti-aging food factors in commercial flavonoids. Hesperidin derived from the Citrus genus extended the lifespan of yeast at doses of 5 and 10 µM as compared with the control group (p<0.01, p<0.01). Reactive oxygen species (ROS), real-time PCR (RT-PCR), and lifespan assays of uth1 and skn7 mutants with the K6001 background were used to study the anti-aging mechanisms in yeast. The results indicate that hesperidin significantly inhibits the ROS of yeast, and UTH1 gene expression, and that SKN7 gene are involved in hesperidin-mediated lifespan extension. Further, increases in the Sir2 homolog, SIRT1 activity, and SOD gene expression were confirmed at doses of 5 (p<0.01) and 10 µM (p<0.05). This suggests that Sir2, UTH1 genes, and ROS inhibition after administration of hesperidin have important roles in the anti-aging effects of yeast. However, the aglycon hesperetin did not exhibit anti-aging effects in yeast.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Superóxido Dismutase
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Espécies Reativas de Oxigênio
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Proteínas de Saccharomyces cerevisiae
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Proteínas Mitocondriais
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Sirtuína 2
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Proteínas de Choque Térmico
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Hesperidina
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article