Fenretinide cytotoxicity is independent of both constitutive and pharmacologically modulated glutathione levels in pediatric acute lymphoblastic leukemia cells cultured at hypoxia.
Pediatr Blood Cancer
; 58(6): 994-7, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22532987
ABSTRACT
Fenretinide (4-HPR) cytotoxicity relative to glutathione levels in pediatric acute lymphoblastic leukemia cell lines cultured at bone marrow level hypoxia (5% O2) is evaluated. 4-HPR cytotoxicity correlated with reactive oxygen species generation (P < 0.001),but not with levels of intracellular glutathione, g-glutamylcysteine synthase, or glutathione peroxidase. Buthionine sulfoximine (BSO)reduced glutathione levels in 10 cell lines (P < 0.001), but 4-HPR þ BSO was markedly synergistic in only 1 of 10 lines. Pretreatment with N-acetylcysteine increased glutathione (P < 0.02)but did not alter 4-HPR cytotoxicity. Our data suggest that 4-HPR cytotoxicity is independent of glutathione under physiologic oxygen tension.
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Base de dados:
MEDLINE
Assunto principal:
Espécies Reativas de Oxigênio
/
Fenretinida
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Glutationa
/
Antineoplásicos
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article