In vitro studies of the influence of glutamatergic agonists on the Na+,K(+)-ATPase and K(+)-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats.
J Negat Results Biomed
; 11: 12, 2012 May 10.
Article
em En
| MEDLINE
| ID: mdl-22574873
ABSTRACT
BACKGROUND:
The overstimulation of excitatory glutamatergic neurotransmission and the inhibition of Na(+),K(+)-ATPase enzymatic activity have both been implicated in neurotoxicity and are possibly related to the pathogenesis of epilepsy and neurodegenerative disorders. In the present study, we investigated whether glutamatergic stimulation by the glutamatergic agonists glutamate, α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA), kainate and N-methyl-d-aspartate (NMDA) modulates the Na(+),K(+)-ATPase and the K(+)-p-nitrophenylphosphatase activities in the crude synaptosomal fraction of the hippocampus and the frontal cortex of rats.RESULTS:
Our results demonstrated that these glutamatergic agonists did not influence the activities of Na(+),K(+)-ATPase or K(+)-p-nitrophenylphosphatase in the brain structures analyzed. Assays with lower concentrations of ATP to analyze the preferential activity of the Na(+),K(+)-ATPase isoform with high affinity for ATP did not show any influence either.CONCLUSIONS:
These findings suggest that under our experimental conditions, the stimulation of glutamatergic receptors does not influence the kinetics of the Na(+),K(+)-ATPase enzyme in the hippocampus and frontal cortex.
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Base de dados:
MEDLINE
Assunto principal:
ATPase Trocadora de Sódio-Potássio
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4-Nitrofenilfosfatase
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Agonistas de Aminoácidos Excitatórios
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Lobo Frontal
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Hipocampo
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article