If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer.
J Mammary Gland Biol Neoplasia
; 17(2): 125-9, 2012 Jun.
Article
em En
| MEDLINE
| ID: mdl-22581273
ABSTRACT
A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Proteínas de Fluorescência Verde
/
Metástase Linfática
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Glândulas Mamárias Animais
/
Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article