Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids.
Methods Mol Biol
; 869: 247-58, 2012.
Article
em En
| MEDLINE
| ID: mdl-22585491
ABSTRACT
Isoelectric focusing (IEF) coupled with immunodetection (immunofixation or immunoblotting) has become the leading technique for the detection and study of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and also is increasingly used in other body fluids such as the tear and serum. Limited commercial availability of precast agarose IEF gels for research and a need for customization prompted reporting a detailed general protocol for the preparation and casting of agarose IEF gel along with sample, control, and isoelectric point marker preparation and carrying out the focusing itself for CSF OCBs. However, the method is readily adaptable to the use of other body fluid specimens and, possibly, research specimens such as culture fluids as well.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bandas Oligoclonais
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article