Galanin expression in the mouse major pelvic ganglia during explant culture and following cavernous nerve transection.
J Mol Neurosci
; 48(3): 713-20, 2012 Nov.
Article
em En
| MEDLINE
| ID: mdl-22585545
ABSTRACT
Autonomic neurons commonly respond to injury/axotomy with an increased expression of neuropeptides including galanin and pituitary adenylyl cyclase-activating polypeptide (PACAP). The increased peptide expression may enhance neuronal survival and axonal regeneration. Using quantitative (Q) PCR and immunocytochemistry, the present study tested whether galanin expression increased in male mouse major pelvic ganglia (MPG) neurons in response to injury. Galanin transcript expression increased significantly in MPG neurons following 72 h in explant culture and 72 h after unilateral transection of the cavernous nerve. Under both conditions, the increase in galanin transcript levels was greater than the increase in PACAP transcript levels. In control MPG, galanin-IR nerve fibers formed pericellular arrangements around MPG neurons although few galanin-IR cells were evident and many of the galanin-IR cells may be small intensely fluorescent (SIF) cells. In 3-day-cultured MPGs, many more galanin-IR cells and nerve fibers were noted. The increased galanin expression was most apparent in neurons that were also immunoreactive for neuronal nitric oxide synthase, rather than tyrosine hydroxylase. Some explant-cultured MPG neurons exhibited immunoreactivity to galanin and PACAP. As reported previously for PACAP, there is an injury-induced increase in MPG galanin expression, which occurs preferentially in the parasympathetic postganglionic neurons.
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Base de dados:
MEDLINE
Assunto principal:
Pênis
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Fibras Parassimpáticas Pós-Ganglionares
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Galanina
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Traumatismos dos Nervos Periféricos
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Gânglios Parassimpáticos
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article